Go to section Main
Go to sectionEducation
Go to sectionResearch
Go to sectionClinic
Ask your question can be via the feedback form.
Go to page
Full list of contact details of departments and heads of departments of the University.
©Collective of authors, 2013
The aim of this study was to evaluate the performance of the PLEX-ID platform based on PCR amplification coupled with electrospray ionization time-of-flight mass spectrometry (PCR/ESI-TOF MS) for detection, identification, and determination of the distribution of pathogenic microorganisms in blood specimens obtained from patients with suspected bloodstream infections. A total of 154 whole blood cultures (74 positive and 80 negative determined by the BacT/Alert 3D instrument, bioMérieux) collected during 3 month period were evaluated using PLEX-ID and the results were compared with those obtained by microbiology culture. PLEX-ID and microbiology culture reached an agreement of 90,8% at the genus and 76% at the species levels in bacterial and candidal identification. Multiple organisms were detected in 10 out of 74 (13.5%) specimens by PLEX-ID, and in 4 (4%) by culture. Time required for bacteria and Candida species identification decreased at least for 24 h and for 48 h, respectively, when using PLEX-ID platform.
Key words: blood culture, PCR/ESI-TOF MS, PLEX-ID, septic patients
Contact: Anna G. Polischouk, Тel.: (812) 303-51-40
© 2014 North-Western State Medical University named after I.I. Mechnikov